Part:BBa_K133004

CF-UB33-RGD-Histop

Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal)fused with UB33. Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production.

UB33 is a peptide derived from the catalytic site of beta subunit of H. pylori urease. Urease is an important pathogenicity factor for H. pylori since it enables the bacteria to breakdown of urea into ammonia and carbon dioxide, thus artificially buffering the acidic enviroment in the stomach.

Since the protein was designed to be produced, we additionally added RGD-Hisstop tag (for the part description and implication follow: [1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1207
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 764
  Illegal BamHI site found at 1275
  Illegal BamHI site found at 1627
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 301
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1721